Effective vascularization is crucial for the success of tissue engineering, influenced by numerous factors. The present work focuses on investigating the effect of a substance, cyanobacteria-loaded oxygen-releasing hydrogel, on vascularization, and verifying the effect of photosynthesis-oxygen-releasing biomaterials containing cyanobacteria hydrogel on angiogenesis, using chick chorioallantoic membrane (CAM) as a model system. On the eighth day of embryonic development, cyanobacterial microspheres were placed on the CAM and kept in a light incubator with appropriate growth and photosynthesis conditions. The effect of cyanobacterial microspheres on vascularization was evaluated from the eighth day of embryonic development. The carrier material used to prepare the microspheres was calcium alginate hydrogel, which is biocompatible to keep embryonic vitality. The article studied the preparation method, optimal process method, and the specific effects of in vivo co-culture on CAM membrane vascularization and development. The data indicates that our prepared photosynthetic oxygen-releasing blue-green algal microspheres have the potential for symbiosis with tissues by supplying oxygen to tissues and inducing vascular growth through photosynthetic oxygen release. This research opens new avenues for applying cyanobacterial microspheres, a novel biological oxygen-releasing material, in regenerative medicine.
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